10.1021/nl1026056.s001
Richard S. Gaster
Richard S.
Gaster
Drew A. Hall
Drew A.
Hall
Shan X. Wang
Shan X.
Wang
Autoassembly Protein Arrays for Analyzing Antibody Cross-Reactivity
American Chemical Society
2015
sensitivity
binding
manner
Autoassembly Protein Arrays
technique
identification
screening
nanosensor
method
15 min
autoassembly protein array
assay
nanoparticle technology
Antibody
50 fM
Analyzing
antibody
2015-12-16 19:25:44
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Autoassembly_Protein_Arrays_for_Analyzing_Antibody_Cross_Reactivity/2016309
We report an autoassembly protein array capable of rapidly screening for aberrant antibody−antigen binding events. Our technique combines magnetic nanoparticle technology with proximity-based, magnetically responsive nanosensors for rapid (under 15 min) and high-density screening of antibody cross-reactivity at sensitivities down to 50 fM in a homogeneous assay. This method will enable the identification of the precise cause of aberrant or cross-reactive binding events in an easy-to-use, rapid, and high-throughput manner.