Bromocriptine as a Novel Pharmacological Chaperone for Mucopolysaccharidosis IV A Olarte-AvellanedaSergio Cepeda Del CastilloJacobo Rojas-RodriguezAndrés Felipe SánchezOscar Rodríguez-LópezAlexander Suárez GarcíaDiego A. PulidoLuz Mary Salazar Alméciga-DíazCarlos J. 2020 Mucopolysaccharidosis IVA (MPS IVA) is a lysosomal storage disease caused by mutations in the gene encoding for the enzyme <i>N</i>-acetylgalactosamine-6-sulfate sulfatase (GALNS), leading to lysosomal accumulation of keratan sulfate (KS) and chondroitin-6-sulfate. In this study, we identified and characterized bromocriptine (BC) as a novel PC for MPS IVA. BC was identified through virtual screening and predicted to be docked within the active cavity of GALNS in a similar conformation to that observed for KS. BC interacted with similar residues to those predicted for natural GALNS substrates. <i>In vitro</i> inhibitory assay showed that BC at 50 μM reduced GALNS activity up to 30%. However, the activity of hrGALNS produced in HEK293 cells was increased up to 1.48-fold. BC increased GALNS activity and reduced lysosomal mass in MPS IVA fibroblasts in a mutation-dependent manner. Overall, these results show the potential of BC as a novel PC for MPS IVA and contribute to the consolidation of PCs as a potential therapy for this disease.