Heo, Dong Nyoung Alioglu, Mecit Altan Wu, Yang Ozbolat, Veli Ayan, Bugra Dey, Madhuri Kang, Youngnam Ozbolat, Ibrahim T. 3D Bioprinting of Carbohydrazide-Modified Gelatin into Microparticle-Suspended Oxidized Alginate for the Fabrication of Complex-Shaped Tissue Constructs Extrusion-based bioprinting of hydrogels in a granular secondary gel enables the fabrication of cell-laden three-dimensional (3D) constructs in an anatomically accurate manner, which is challenging using conventional extrusion-based bioprinting processes. In this study, carbohydrazide-modified gelatin (Gel-CDH) was synthesized and deposited into a new multifunctional support bath consisting of gelatin microparticles suspended in an oxidized alginate (OAlg) solution. During extrusion, Gel-CDH and OAlg were rapidly cross-linked because of the Schiff base formation between aldehyde groups of OAlg and amino groups of Gel-CDH, which has not been demonstrated in the domain of 3D bioprinting before. Rheological results indicated that hydrogels with lower OAlg to Gel-CDH ratios possessed superior mechanical rigidity. Different 3D geometrically intricate constructs were successfully created upon the determination of optimal bioprinting parameters. Human mesenchymal stem cells and human umbilical vein endothelial cells were also bioprinted at physiologically relevant cell densities. The presented study has offered a novel strategy for bioprinting of natural polymer-based hydrogels into 3D complex-shaped biomimetic constructs, which eliminated the need for cytotoxic supplements as external cross-linkers or additional cross-linking processes, therefore expanding the availability of bioinks. OAlg;Rheological results;Different 3 D;bioprinting parameters;polymer-based hydrogels;Schiff base formation;3 D Bioprinting;novel strategy;3 D bioprinting;cross-linking processes;Human mesenchymal;aldehyde groups;Carbohydrazide-Modified Gelatin;carbohydrazide-modified gelatin;3 D complex-shaped biomimetic;Complex-Shaped Tissue Constructs Extrusion-based bioprinting;Microparticle-Suspended Oxidized Alginate;cell densities;Gel-CDH ratios;multifunctional support bath;cytotoxic supplements;umbilical vein;extrusion-based bioprinting processes;gelatin microparticles 2020-04-21
    https://acs.figshare.com/articles/journal_contribution/3D_Bioprinting_of_Carbohydrazide-Modified_Gelatin_into_Microparticle-Suspended_Oxidized_Alginate_for_the_Fabrication_of_Complex-Shaped_Tissue_Constructs/12170235
10.1021/acsami.0c05096.s001