%0 Journal Article %A Sun, Zhaowei %A Wang, Lei %A Wu, Shuai %A Pan, Yanhong %A Dong, Yu %A Zhu, Sha %A Yang, Jie %A Yin, Yongmei %A Li, Genxi %D 2020 %T An Electrochemical Biosensor Designed by Using Zr-Based Metal–Organic Frameworks for the Detection of Glioblastoma-Derived Exosomes with Practical Application %U https://acs.figshare.com/articles/journal_contribution/An_Electrochemical_Biosensor_Designed_by_Using_Zr-Based_Metal_Organic_Frameworks_for_the_Detection_of_Glioblastoma-Derived_Exosomes_with_Practical_Application/11857968 %R 10.1021/acs.analchem.9b05241.s001 %2 https://acs.figshare.com/ndownloader/files/21732936 %K GBM-derived exosomes %K Practical Application Glioblastoma %K epidermal growth factor receptor %K MOF %K BBB %K EGFR %K III %K diagnosis %K label-free electrochemical biosensor %X Glioblastoma (GBM) is one of the most fatal tumors in the brain, and its early diagnosis remains technically challenging due to the complex repertoires of oncogenic alterations and blood-brain barrier (BBB). GBM-derived specific exosomes can cross the BBB and circulate in body fluids, so they can be noninvasive biomarkers for the early diagnosis of GBM. Herein, we propose a sensitive and label-free electrochemical biosensor designed by using Zr-based metal–organic frameworks (Zr-MOFs) for the detection of GBM-derived exosomes with practical application. In the design, a peptide ligand can specifically bind with human epidermal growth factor receptor (EGFR) and EGFR variant (v) III mutation (EGFRvIII), which are overexpressed on the GBM-derived exosomes. Meanwhile, Zr-MOFs encapsulated with methylene blue can absorb on the surface of the exosomes due to the interaction between Zr4+ and the intrinsic phosphate groups outside of exosomes. Consequently, the concentration of exosomes can be directly quantified by monitoring the electroactive molecules inside MOFs, ranging from 9.5 × 103 to 1.9 × 107 particles/μL with the detection of limit of 7.83 × 103 particles/μL. Furthermore, this proposed biosensor can distinguish GBM patients from healthy groups, demonstrating the great prospect for early clinical diagnosis. %I ACS Publications