10.1021/acsabm.9b00885.s001
Meng-Yu Tsai
Meng-Yu
Tsai
Fumihiro Aratsu
Fumihiro
Aratsu
Shogo Sekida
Shogo
Sekida
Shingo Kobayashi
Shingo
Kobayashi
Masaru Tanaka
Masaru
Tanaka
Blood-Compatible Poly(2-methoxyethyl acrylate) Induces
Blebbing-like Phenomenon and Promotes Viability of Tumor Cells in
Serum-Free Medium
American Chemical Society
2020
Induces Blebbing-like Phenomenon
CTC
PMEA substrate
HT
Serum-Free Medium Circulating tumor cells
cell viability
2020-02-11 11:43:50
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Blood-Compatible_Poly_2-methoxyethyl_acrylate_Induces_Blebbing-like_Phenomenon_and_Promotes_Viability_of_Tumor_Cells_in_Serum-Free_Medium/11836077
Circulating tumor cells (CTCs) are
highly related to tumor metastasis
and an effective technique of detecting/isolating CTCs has been expected
to be established for tumor treatments. Previously, we reported that
platelets cannot adhere but tumor cells can adhere on poly(2-methoxyethyl
acrylate) (PMEA) substrate. In this study, we report that cell viability
of human fibrosarcoma (HT-1080) cells was promoted on PMEA substrate
in serum-free medium. The significant blebbing-like phenomenon and
spontaneous formation of cell aggregation were observed on PMEA substrate.
Moreover, cell viability was promoted by activation of Akt signaling
pathway via N-cadherin-mediated cell–cell contact. These results
suggest that not only capture efficiency and purity but also high
cell viability of CTCs can be accomplished by using PMEA substrate.
PMEA substrate can be a promising candidate for medical applications
such as in vitro screening of anticancer drugs and is an excellent
platform for studies and diagnoses of tumor migration and metastasis.