10.1021/acsabm.9b00885.s001 Meng-Yu Tsai Meng-Yu Tsai Fumihiro Aratsu Fumihiro Aratsu Shogo Sekida Shogo Sekida Shingo Kobayashi Shingo Kobayashi Masaru Tanaka Masaru Tanaka Blood-Compatible Poly(2-methoxyethyl acrylate) Induces Blebbing-like Phenomenon and Promotes Viability of Tumor Cells in Serum-Free Medium American Chemical Society 2020 Induces Blebbing-like Phenomenon CTC PMEA substrate HT Serum-Free Medium Circulating tumor cells cell viability 2020-02-11 11:43:50 Journal contribution https://acs.figshare.com/articles/journal_contribution/Blood-Compatible_Poly_2-methoxyethyl_acrylate_Induces_Blebbing-like_Phenomenon_and_Promotes_Viability_of_Tumor_Cells_in_Serum-Free_Medium/11836077 Circulating tumor cells (CTCs) are highly related to tumor metastasis and an effective technique of detecting/isolating CTCs has been expected to be established for tumor treatments. Previously, we reported that platelets cannot adhere but tumor cells can adhere on poly­(2-methoxyethyl acrylate) (PMEA) substrate. In this study, we report that cell viability of human fibrosarcoma (HT-1080) cells was promoted on PMEA substrate in serum-free medium. The significant blebbing-like phenomenon and spontaneous formation of cell aggregation were observed on PMEA substrate. Moreover, cell viability was promoted by activation of Akt signaling pathway via N-cadherin-mediated cell–cell contact. These results suggest that not only capture efficiency and purity but also high cell viability of CTCs can be accomplished by using PMEA substrate. PMEA substrate can be a promising candidate for medical applications such as in vitro screening of anticancer drugs and is an excellent platform for studies and diagnoses of tumor migration and metastasis.