Artepillin C, a Key Component of Brazilian Propolis, Induces Thermogenesis in Inguinal White Adipose Tissue of Mice through a Creatine-Metabolism-Related Thermogenic Pathway Sho Nishikawa Takuma Hydo Hiroki Aoyama Ryo Miyata Shigenori Kumazawa Takanori Tsuda 10.1021/acs.jafc.9b07080.s001 https://acs.figshare.com/articles/journal_contribution/Artepillin_C_a_Key_Component_of_Brazilian_Propolis_Induces_Thermogenesis_in_Inguinal_White_Adipose_Tissue_of_Mice_through_a_Creatine-Metabolism-Related_Thermogenic_Pathway/11652327 Induction of beige adipocytes in white adipose tissue (WAT) is a potential therapeutic target for the treatment of obesity because beige adipocytes release excess energy via uncoupling-protein-1-associated thermogenesis. In this study, we investigated how artepillin C (ArtC) promotes thermogenesis <i>in vivo</i>. We demonstrated that 28 day administration of ArtC (10 mg/kg of body weight) to mice significantly induced thermogenesis in beige adipocytes in inguinal WAT (iWAT) and suppressed reductions in core body temperature induced by cold exposure at 4 °C. Moreover, ArtC-induced thermogenesis in iWAT was significantly inhibited by treatment with a creatine metabolism inhibitor, and ArtC significantly upregulated the expression of creatine-metabolism-related enzymes in the thermogenic pathway. These results indicate that ArtC induces thermogenesis in beige adipocytes in iWAT, and the observed ArtC-induced thermogenesis is associated with the creatine-metabolism-related thermogenic pathway, which is characteristically observed in beige adipocytes. 2020-01-18 20:13:43 creatine-metabolism-related thermogenic pathway Inguinal White Adipose Tissue WAT core body temperature creatine metabolism inhibitor uncoupling-protein -1-associated thermogenesis iWAT ArtC-induced thermogenesis Creatine-Metabolism-Related Thermogenic Pathway Induction adipocyte 28 day administration