%0 Journal Article %A Tang, Xiaoqian %A Wu, Jing %A Wu, Wenqin %A Zhang, Zhaowei %A Zhang, Weiqi %A Zhang, Qi %A Zhang, Wen %A Chen, Xiaomei %A Li, Peiwu %D 2020 %T Competitive-Type Pressure-Dependent Immunosensor for Highly Sensitive Detection of Diacetoxyscirpenol in Wheat via Monoclonal Antibody %U https://acs.figshare.com/articles/journal_contribution/Competitive-Type_Pressure-Dependent_Immunosensor_for_Highly_Sensitive_Detection_of_Diacetoxyscirpenol_in_Wheat_via_Monoclonal_Antibody/11652309 %R 10.1021/acs.analchem.9b03933.s001 %2 https://acs.figshare.com/ndownloader/files/21156207 %K concentration %K matrix-matched calibration curves %K Competitive-Type Pressure-Dependent Immunosensor %K goat antimouse antibody %K wheat samples %K DAS %K competitive-type pressure-dependent immunosensor %K CTPDI %K chromatography-tandem mass spectrometry %K Monoclonal Antibody Diacetoxyscirpenol %K IC 50 value %X Diacetoxyscirpenol (DAS) is a type A trichothecene mycotoxin with low molecular weight, and with respect to its toxicity and the occurrence in food and feed, it is known as a potential risk for public and animal health. In the present study, first, a sensitive and specific monoclonal antibody (5E7) was developed. Then, the antibody was applied to develop a competitive-type pressure-dependent immunosensor (CTPDI). The Au@PtNP was synthesized and labeled with goat antimouse antibody (Au@PtNPs-IgG). Finally, the concentration of DAS was negatively correlated with the pressure signal. In the presence of optimal conditions, matrix-matched calibration curves were plotted for wheat samples, in which an optimal IC50 value (half maximal inhibitory concentration) of 3.08 ng/g was achieved. The CTPDI was further applied to detect natural and blind wheat samples, and validation was carried out by liquid chromatography-tandem mass spectrometry. The results showed that CTPDI was highly appropriate and accurate for detection of DAS in wheat. %I ACS Publications