%0 Online Multimedia
%A Vinagreiro, Carolina S.
%A Zangirolami, Amanda
%A Schaberle, Fabio A.
%A Nunes, Sandra C. C.
%A Blanco, Kate C.
%A Inada, Natalia M.
%A da Silva, Gabriela Jorge
%A Pais, Alberto A. C. C.
%A Bagnato, Vanderlei S.
%A Arnaut, Luis G.
%A Pereira, Mariette M.
%D 2020
%T Antibacterial Photodynamic Inactivation of Antibiotic-Resistant
Bacteria and Biofilms with Nanomolar Photosensitizer Concentrations
%U https://acs.figshare.com/articles/media/Antibacterial_Photodynamic_Inactivation_of_Antibiotic-Resistant_Bacteria_and_Biofilms_with_Nanomolar_Photosensitizer_Concentrations/11632317
%R 10.1021/acsinfecdis.9b00379.s003
%2 https://acs.figshare.com/ndownloader/files/21093021
%K 6.9 log units
%K Nanomolar Photosensitizer Concentrations
%K drug diffusion
%K cell death mechanisms
%K 5 nM photosensitizer concentration
%K antibiotic-resistant bacteria
%K charge distribution
%K photosensitizer drug impacts
%K Antibacterial Photodynamic Inactivation
%K nosocomial infections
%K imidazolyl porphyrins
%K Antibiotic-Resistant Bacteria
%K Antibacterial photodynamic inactivation
%K PDI
%K Therapeutic alternatives
%K oxidative stress
%K biofilm infections
%X Gram-negative
bacteria and bacteria in biofilms are very difficult to eradicate
and are the most antibiotic-resistant bacteria. Therapeutic alternatives
less susceptible to mechanisms of resistance are urgently needed to
respond to an alarming increase of resistant nosocomial infections.
Antibacterial photodynamic inactivation (PDI) generates oxidative
stress that triggers multiple cell death mechanisms that are more
difficult to counteract by bacteria. We explore PDI of multidrug-resistant
bacterial strains collected from patients and show how positive charge
distribution in the photosensitizer drug impacts the efficacy of inactivation.
We demonstrate the relevance of size for drug diffusion in biofilms.
The designed meso-imidazolyl porphyrins of small
size with positive charges surrounding the macrocycle enabled the
inactivation of bacteria in biofilms by 6.9 log units at 5 nM photosensitizer
concentration and 5 J cm–2, which offers new opportunities
to treat biofilm infections.
%I ACS Publications