10.1021/acs.analchem.9b03021.s002
Peixian Li
Peixian
Li
Minhui Liang
Minhui
Liang
Xiaoguang Lu
Xiaoguang
Lu
Joycelyn Jia Ming Chow
Joycelyn Jia Ming
Chow
Chrishan J. A. Ramachandra
Chrishan J. A.
Ramachandra
Ye Ai
Ye
Ai
Sheathless
Acoustic Fluorescence Activated Cell Sorting
(aFACS) with High Cell Viability
American Chemical Society
2019
cell level
High Cell Viability
FTSAW
Sheathless Acoustic Fluorescence Activated Cell
MDA
aFACS system
recovery rate
1 μ m particles
human-induced pluripotent stem-cell-derived cardiomyocytes
MCF
cell viability
2019-11-18 20:34:51
Media
https://acs.figshare.com/articles/media/Sheathless_Acoustic_Fluorescence_Activated_Cell_Sorting_aFACS_with_High_Cell_Viability/10324451
In this work, we demonstrate a sheathless acoustic fluorescence
activated cell sorting (aFACS) system by combining elasto-inertial
cell focusing and highly focused traveling surface acoustic wave (FTSAW)
to sort cells with high recovery rate, purity, and cell viability.
The microfluidic sorting device utilizes elasto-inertial particle
focusing to align cells in a single file for improving sorting accuracy
and efficiency without sample dilution. Our sorting device can effectively
focus 1 μm particles which represents the general minimum size
for a majority of cell sorting applications. Upon the fluorescence
interrogation at the single cell level, individual cells are deflected
to the target outlet by a ∼50 μm wide highly focused
acoustic field. We have applied our aFACS to sort three different
cell lines (i.e., MCF-7, MDA-231, and human-induced pluripotent stem-cell-derived
cardiomyocytes; hiPSC-CMs) at ∼kHz with a sorting purity and
recovery rate both of about 90%. A further comparison demonstrates
that the cell viability drops by 35–45% using a commercial
FACS machine, while the cell viability only drops by 3–4% using
our aFACS system. The developed aFACS system provides a benchtop solution
for rapid, highly accurate single cell level sorting with high cell
viability, in particular for sensitive cell types.