Novel Dextransucrase Gtf-DSM, Highly Similar in Sequence
to Reuteransucrase GtfO, Displays Unique Product Specificity
Ziwei Chen
Yuqing Tian
Wenli Zhang
Cuie Guang
Xiangfeng Meng
Wanmeng Mu
10.1021/acs.jafc.9b04252.s001
https://acs.figshare.com/articles/journal_contribution/Novel_Dextransucrase_Gtf-DSM_Highly_Similar_in_Sequence_to_Reuteransucrase_GtfO_Displays_Unique_Product_Specificity/10274831
Lactic
acid bacteria are known to produce extracellular polysaccharides
such as α-glucan, levan, and inulin, which are promising for
applications in food systems because of their prebiotic properties.
In this work, a novel glucansucrase (GS) Gtf-DSM from Lactobacillus ingluviei DSM 14792 was recombinantly
expressed and biochemically characterized as a dextransucrase capable
of producing a dextran polysaccharide with four types of linkages,
including 69% (α1 → 6), 24% (α1 → 3), 6%
(α1 → 4), and 1% (α1 → 2). Intriguingly,
the dextransucrase Gtf-DSM had a sequence identity of 99.3% with the
reuteransucrase GtfO producing a reuteran with 21% (α1 →
6) and 79% (α1 → 4) linkages. Thus, the dextransucrase
Gtf-DSM is a unique target for understanding the linkage specificity
of GSs and the investigation of site-directed mutagenesis using Gtf-DSM
and GtfO as templates is underway.
2019-11-08 14:46:14
dextransucrase Gtf-DSM
GtfO
Product Specificity Lactic acid bacteria
Lactobacillus ingluviei DSM 14792
GS
linkage
Novel Dextransucrase Gtf-DSM
polysaccharide