10.1021/acsami.9b15349.s001 Jingjing Shen Jingjing Shen Lei Zhang Lei Zhang Li Liu Li Liu Bin Wang Bin Wang Jieqiong Bai Jieqiong Bai Chao Shen Chao Shen Yu Chen Yu Chen Quli Fan Quli Fan Shufen Chen Shufen Chen Weibing Wu Weibing Wu Xiaomiao Feng Xiaomiao Feng Lianhui Wang Lianhui Wang Wei Huang Wei Huang Revealing Lectin–Sugar Interactions with a Single Au@Ag Nanocube American Chemical Society 2019 surface plasmon resonance biosensor system dark-field microscopy images novel plasmonic nanobiosensors detection monitoring nanobiosensor system NC trace concanavalin biosensing ability trace ConA nanoparticle surface LSPR spectra nanoparticle-based plasmonic nanotechnology lectin 2019-10-18 21:29:47 Journal contribution https://acs.figshare.com/articles/journal_contribution/Revealing_Lectin_Sugar_Interactions_with_a_Single_Au_Ag_Nanocube/10005470 An individual nanoparticle-based plasmonic nanotechnology was used for real-time monitoring of lectin–sugar interactions, which could be designed as novel plasmonic nanobiosensors for the detection of trace concanavalin A (ConA) with high sensitivity and selectivity. The localized surface plasmon resonance (LSPR) spectra of Au@Ag nanocubes (NCs) are linearly shifted to a long wavelength with an increasing concentration of ConA. In fact, each Au@Ag NC can act as a nanobiosensor for the quantified detection of trace ConA, which enables the miniaturization of the biosensor system to nanoscale. Furthermore, the results demonstrated the perfect biosensing ability with the dual channel of dark-field microscopy images and LSPR spectra. We expect that this nanobiosensor system can provide an alternative important method for monitoring the specific binding of lectin–sugar at a single nanoparticle surface.